Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: Stromal vascular fraction (SVF)-derived lymphatic endothelial progenitor cells (LEPCs) show better differentiation by flow cytometry analysis than bone marrow (BM)-derived LEPCs. (A) Flow cytometry workflow of the SVF-derived LEPCs from the first population until the PDPN-positive population. (A.I) PDPN expression histograms showing the expression level for SVF-LEPCs and -MSCs. (A.II) Median fluorescence intensity (MFI) of PDPN in SVF-LEPCs and –MSCs (mean ± SD). (B) Flow cytometry workflow of the BM-derived LEPCs from the first population until the PDPN-positive population (B.I) PDPN expression histograms showing the expression level for BM-LEPCs and -MSCs. (B.II) MFI of PDPN in BM-LEPCs and –MSCs (mean ± SD). Statistical significance: * p -value<0.5, ** p -value<0.01. Mann-Whitney test. n=6 for SVF-LEPCs and n=5 for BM-LEPCs. Mean ± SD represented.

Article Snippet: Human dermal lymphatic endothelial cells (HDLEC, C-14021, PromoCell) were cultured according to the providers’ guidelines.

Techniques: Derivative Assay, Flow Cytometry, Expressing, Fluorescence, MANN-WHITNEY

Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: Characterization of lymphatic endothelial progenitor cells (LEPCs) shows correct differentiation by overexpression of lymphatic lineage specific markers. (A) RT-qPCR shows statistically significant overexpression of key lymphatic markers such as Pdpn , Vegfr3 , Lyve1 , Vegf-c and Igf2 . n=4. MSC, mesenchymal stem cells. Mean ± SD. (B) Median fluorescence intensity analysis of immunofluorescence staining images shows statistically significant differential expression of PDPN between MSCs and LEPCs. Scale bar: 100 µm. n=6. Statistical significance: * p -value<0.05; ** p -value<0.01. Mann-Whitney test.

Article Snippet: Human dermal lymphatic endothelial cells (HDLEC, C-14021, PromoCell) were cultured according to the providers’ guidelines.

Techniques: Over Expression, Quantitative RT-PCR, Fluorescence, Immunofluorescence, Staining, Quantitative Proteomics, MANN-WHITNEY

Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: Stromal vascular fraction (SVF)-derived lymphatic endothelial progenitor cells (LEPCs) show a reliable lymphatic phenotype in vitro. (A) Genotype stability results of the key overexpressed genes over passages (P), assessed by RT-qPCR. n=3. (B) Scratch-wound assay of the SVF-derived LEPCs in comparison to human dermal lymphatic endothelial cells (HDLECs) along two days in culture. n=3. Scale bar: 200 µm. Mean ± SD. Mann-Whitney test.

Article Snippet: Human dermal lymphatic endothelial cells (HDLEC, C-14021, PromoCell) were cultured according to the providers’ guidelines.

Techniques: Derivative Assay, In Vitro, Quantitative RT-PCR, Scratch Wound Assay Assay, Comparison, MANN-WHITNEY

Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: Stromal vascular fraction (SVF)-derived lymphatic endothelial progenitor cells (LEPCs) show better in vitro angiogenesis phenotype than human dermal lymphatic endothelial cells (HDLEC). (A) LIVE/DEAD staining images of SVF-derived LEPCs and HDLECs culture on a Matrigel (3 mg/mL) coating for 5 hours (2D angiogenesis assay). (B) Angiogenesis analysis of SVF-derived LEPCs compared to HDLECs in a 2D environment. N=5. (C) Bright field images of SVF-derived LEPCs and HDLECs embedded in a 3D Matrigel hydrogel (3 mg/mL) and cultured for 10 days. (D) LIVE/DEAD staining images of SVF-derived LEPCs and HDLECs embedded in a 3D Matrigel hydrogel (3 mg/mL) and cultured for 10 days. (E) Angiogenesis analysis of SVF-derived LEPCs compared to HDLECs in a 3D environment. n=4. Scale bars: (A) 500 µm; (C-D) 200 µm. Statistical significance: * p -value<0.05. Mann-Whitney test.

Article Snippet: Human dermal lymphatic endothelial cells (HDLEC, C-14021, PromoCell) were cultured according to the providers’ guidelines.

Techniques: Derivative Assay, In Vitro, Staining, Angiogenesis Assay, Cell Culture, MANN-WHITNEY

Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: Stromal vascular fraction (SVF)-derived lymphatic endothelial progenitor cells (LEPCs) express canonical lymphatic markers in a 3D environment. Representative immunofluorescence staining images of LEPCs expressing lymphatic markers (PDPN, LYVE1, VEGFR3 and F-actin) embedded in Matrigel for 10 days. To the right, magnification of the white square in the left. Scale bar: 100 µm.

Article Snippet: Human dermal lymphatic endothelial cells (HDLEC, C-14021, PromoCell) were cultured according to the providers’ guidelines.

Techniques: Derivative Assay, Immunofluorescence, Staining, Expressing

Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: RNA sequencing analysis of Stromal vascular fraction (SVF)-derived lymphatic endothelial progenitor cells (LEPCs) confirm lymphatic genotype in comparison to mesenchymal progenitor cells. (A) Variance-stabilized transformed (VST) counts were used for Principal Component Analysis (PCA) plot. Axes show the top two principal components (PC1: 69.2%, PC2: 9.3%). Samples are colored by condition. (B) Sample clustering of the top 500 most variable genes. Distances between genes were estimated based on spearman correlation, which were then used to produce a clustering via the ward.D2 method. (C) Volcano plot of the differentially expressed genes (DEGs) between LEPCs and MSCs. Gray: non-significant; red: significant DEGs (|log2FC|≥1, adjusted p -value<0.05). (D) Boxplot of the differential expression of canonic lymphatic markers. Significance: ***adjusted p -value<0.001 (DESeq2 Wald test). (E) Heatmap of top 50 lymphatic endothelial cell (LEC) markers. Z-score-scaled VST counts for the 50 most significant LEC markers (rows) across samples (columns). Bold genes: |log2 fold change|≥1, adjusted p -value≤0.05. Clustering uses Euclidean distance and Ward.D2 linkage.

Article Snippet: Human dermal lymphatic endothelial cells (HDLEC, C-14021, PromoCell) were cultured according to the providers’ guidelines.

Techniques: RNA Sequencing, Derivative Assay, Comparison, Transformation Assay, Quantitative Proteomics

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: In vitro characterization of new anti-CEA antibodies. (a) SPR sensorgram of G9, F7, and F4 in a scFv format. K D values were calculated to be 640 nM, 50 nM, and 7.7 nM, respectively. (b) Flow cytometry analysis with the new antibodies in IgG format on CEA-expressing CT26 cells and on CEA-negative CT26 wild-type cells. (c) Immunofluorescence staining with IgG formats on the human colon adenocarcinoma xenograft LS174T. Anti-CEA antibodies were detected in green. Blood vessels were detected by CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: In Vitro, Flow Cytometry, Expressing, Immunofluorescence, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Ex vivo immunofluorescence-based biodistribution analysis. Immunofluorescence analysis assessed tumor targeting of new anti-CEA antibodies in IgG format. Two hundred micrograms of IgG-FITC were injected intravenously into LS174T-bearing mice. Tumors were excised 24 hours after injection. IgG-FITC was detected in green; blood vessels were detected through CD31 staining (red). 20× magnification, scale bars = 100 μm.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Ex Vivo, Immunofluorescence, Injection, Staining

Journal: mAbs

Article Title: Generation and in vivo characterization of a novel high-affinity human antibody targeting carcinoembryonic antigen

doi: 10.1080/19420862.2023.2217964

Figure Lengend Snippet: Quantitative biodistribution with radiolabeled anti-CEA antibodies in diabody format. Quantitative biodistribution analysis of radio iodinated anti-CEA diabodies in BALB/c nude mice bearing subcutaneous LS174T colon adenocarcinomas. Organs were harvested 24 hours after intravenous injection, and radioactivity was quantified. Results are shown as the percentage of injected dose per gram (ID/g (%)). Error bars = SEM; n = 4.

Article Snippet: Immunofluorescence analysis was performed on LS174T and HT-29 xenografts harvested from mice, on patient-derived colon cancer samples, and on a human tissue microarray (Amsbio, T6235700–5).

Techniques: Injection, Radioactivity